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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so may perhaps add to tumorigenesis. This protein was found to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a essential part in marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not yet readily available.
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Blocking peptides are peptides that bind exclusively into the target antibody and block antibody binding. These peptide normally has the epitope recognized with the antibody.
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This guideline outlines further more doable optimization of cellular and immunoassay parameters to ensure the very best outcomes are...
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This manual outlines further feasible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the focus on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, you can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins exhibit distinctive expression and degradation patterns which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and functions as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S changeover. This protein accumulates within the G1-S phase boundary and is degraded as cells development by S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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